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Formalin-fixed rat livers were embedded in paraffin, cut into 5-μm section and stained with Sirius Red according to standard procedures [37]. The area of collagen deposition, as determined by the percentage of the area positive for Sirius Red, was quantified by ImageJ software from the histological images.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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