Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
qRT-PCR Analysis for Evolution and Validation of Candidate Serum Exosomal miRNA
Ask a
question
Favorite
The candidate miRNAs were further confirmed with quantitative real-time polymerase chain reaction (qRT-PCR). qRT-PCR was performed using the QuantiFast® SYBR® Green PCR Master Mix in an ABI 9700 qPCR system (Applied Biosystems, Foster City, CA, USA). The relative gene expression values of the target miRNAs were normalized to that of RNU6B (U6) and the differences were calculated using the 2−∆∆Ct method.25
Copyright and License information: ©2021 Wang et al.
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
