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2.7. Caspase-3/7 Activity Assay

YF Yosuke Fujita
TN Tomoki Nagakura
HU Hiroyuki Uchino
MI Masato Inazu
TY Tsuyoshi Yamanaka
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The hNSCs were seeded on 24-multiwell plates coated with the defined substrate (diluted 1:100, CELLstart, Gibco, USA). HC-3 was added 48 h after hNSC plating, and the final culture medium in each well was 1.0 mL. All culture media and HC-3 were changed every day. For measuring the Caspase-3/7 activity, the Caspase-3/7 assay kit (Caspase-Glo 3/7 Assay System, Promega, Madison, WI, USA) was used. This kit is based on the cleavage of the Z-DEVD-Aminoluciferin sequence of a luminogenic substrate by Caspase-3/7, resulting in a luminescent signal. Their luminescence was measured using a microplate reader (FilterMax F5, Molecular Devices, USA).

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