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4.4. Relative Water Content (RWC) of Leaves
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RWC was determined according to Gao et al. 2019 [28]. Leaves were weighed immediately to obtain fresh weight (FW), soaked in water overnight in the dark and weighed again to obtain turgid fresh weight (TW), and then dried at 75 °C until constant weight (DW). RWC was then calculated as follows:
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