4.3. MTT Assay for Cell Viability

Cells were seeded in a 96-well plate one day prior to treatment with IAld or I3AA. RAW cells and HUVEC were treated for 24 h. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was then added in accordance with the protocol for MTT Assay for Cell Viability and Proliferation (Millipore Sigma). Absorbance was read at 595 nm after 4 h using a Bio-Rad iMark reader. The absorbance relative to the vehicle control was reported (Mean ± SEM) and correlates with relative cell viability [58].