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2.3.4. Automated Data Evaluation Work-Flow

CC Carolina Carrillo-Najar
DR Daniel Rembao-Bojórquez
MT Martha L. Tena-Suck
SZ Sergio Zavala-Vega
NG Noemí Gelista-Herrera
MR Miguel A. Ramos-Peek
JG Juan L. Gómez-Amador
FC Febe Cazares-Raga
FH Fidel de la Cruz Hernández-Hernández
AO Alma Ortiz-Plata
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Tandem mass spectra were extracted in Proteome Discoverer v1.4 and searched against a database on a Sequest HT engine. Searches were executed with the following parameters: 2 Da parent MS ion window, 1 Da MS/MS ion window, and two missed cleavages allowed. The iodoacetamide derivative of cysteine (carbamidomethylcysteine) was specified on Sequest as a fixed modification and oxidation of methionine as a variable modification.

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