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CAMYEL, a cAMP sensor using YFP-Epac-RLuc [23] was utilized to quantify cAMP accumulation with the temporal resolution. Then, 500 ng of CAMYEL DNA was co-transfected with 500 ng of DNA of untagged GLP-1R or GIPR per well in a 6-well plate. The experiments were performed in the absence of 3-isobutyl-1-methylxanthine (IBMX).
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This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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