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The myeloperoxidase (MPO) colorimetric activity assay kit (K744-100, Biovision, US) was used to measure MPO activity according to kit instructions. MPO activity unit was defined as the amount of the enzyme that catalyzes the degradation of 1 nmol of H2O2 per gram of tissue at 37°C. The activity was calculated as MPO=(absorbance of the experimental tube-absorbance of the control tube)/11.3×sample amount. MPO activity reflects the degree of neutrophil accumulation in the lung tissue.

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