4.10. Cell Viability Assay

Cell proliferation assay was performed on ibrutinib-treated SKNBE2, IMR32, SKNSH, SH-SY-5Yand NB9464 cells using alamarBlue^® (Roche) reagent according to the manufacturer’s protocol. Briefly, 1 × 10⁴ cells were seeded in 96-well plates, followed by the addition of ibrutinib at different concentrations. AlamarBlue^® was added after 48 h, and plates were incubated for another 4 h at 37 °C in 5% CO₂. Changes in cell viability are detected using Tecan Infinite F200 proplate reader (Tecan, Mannedorf, Switzerland) and fluorescence signals were read as emission at 590 nm after excitation at 560 nm.