3.2.2. Nrf2/ARE Luciferase Assay

HepG2 cells were seeded in a 6-well culture plate in antibiotic-free medium for 24 h, followed by transfection with an ARE reporter vector that encodes for firefly luciferase in the presence of Lipofectamine 3000 (Invitrogen, San Diego, CA, USA) according to the manufacturer’s instruction. The cells were then treated with different concentrations of compound 1g for 6 h. Luciferase Assay Reagent II (Promega, Madison, WI, USA) was added to determine the activity of firefly luciferase.