2.5. Phage DNA Extraction

DNA extraction was performed by incubating 1 mL of purified phage lysate (10¹⁰ PFU/mL) with DNase I (final concentration 1 ug/mL) and RNase A (final concentration 30 ug/mL) to remove host nucleic acid contamination. After 30 min of incubation at room temperature, the mixture was heated at 75 °C for 5 min to inactivate DNase I. Then, the mixture was treated with 4 µL of proteinase K (20 mg/mL) and lysis buffer B (Phage DNA isolation kit) for 1 h at 56 °C. DNA was further extracted using the Phage DNA isolation kit (Norgen, Biotek, Thorold, ON, Canada) according to the manufacturer’s instructions. The extracted phage DNA was subsequently sequenced.