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4.5. Plant Mineralization and Mineral Element Analysis
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Aliquots of 0.5 g of the air-dried roots or leaves were acid digested in a mixture of 6 mL of 60% HNO3 and 1 mL of 30% H2O2 at 80 °C for 40 min. The digestate was filtered and brought up to 50 mL with ultrapure water (Merk-Millipore Aquatron). The mineral elements were detected by Multi-element measurement in the (ICP-OES) instrument “Inductively Coupled Plasma” Varian Vista [35].
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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