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Bronchoalveolar Lavage (BAL) and Tissue Harvest

AS Angela Rico de Souza
HT Hussein Traboulsi
XW Xinyu Wang
JF Jorg H. Fritz
DE David H. Eidelman
CB Carolyn J. Baglole
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Lungs were excised and PBS (0.5 ml) was injected twice to lavage the lungs. The BAL was centrifuged and the supernatant separated from the cells. The cells were resuspended in PBS, counted, mounted onto slides using a CytoSpin (Thermo Scientific; Waltham, MA) and stained with Three Step Stain (Thermo Scientific, Waltham, MA). Lung tissue was collected and either frozen immediately in liquid nitrogen and stored at −80°C for protein/western blot analysis or stored in RNAlater® (Qiagen) for subsequent mRNA analysis.

Copyright and License information:  ©2021 Rico de Souza, Traboulsi, Wang, Fritz, Eidelman and Baglole.
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