2.6.4. Evaluation of Platelet Viability by MTT Assay

MTT colorimetric assay was performed to assess the platelet viability. Washed platelets (1 × 10⁶ cells/mL) were taken separately in 96-well microtiter plates. We treated the platelets with different concentrations of diluted AgNPs, specifically 0.5, 1, 1.5, 2, and 2.5 µg/µL, with 1× PBS followed by incubation for 2 h at 37 °C. Then after the incubation, 250 μM of MTT (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was added and incubated for an additional 3 h. H₂O₂ was taken as the positive control. Thereafter, MTT was removed, and remaining formazan crystals were completely dissolved in DMSO and the absorbance was recorded at 570 nm using a Varioskan multimode plate reader (Thermofischer scientific, Waltham, MA, USA).