4.5.6. Intra-Assay and Inter-Assay Variability

The intra-assay variability (repeatability or precision within a plate or run) was calculated by testing plasmid controls at concentrations of 100 and 10,000 copies/µL, in four replicates each, on a single plate. The inter-assay repeatability or precision between plates and runs was calculated by running the six plasmid controls at various dilutions (100, 500, 1000, and 10,000 copies/µL) in four different plates each run on four different days. The percent of coefficient of variation (%CV) for the intra-assay and inter-assay variability was determined by dividing the standard deviation of the replicates by the mean, then multiplied by 100.