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Seroepidemiological surveys and statistical analysis

LT Li Tingting
LW Li Wenhui
ZN Zhang Nianzhang
QZ Qu Zigang
JO John A Ohiolei
LL Li Li
YH Yan Hongbin
JW Jia Wanzhong
FB Fu Baoquan
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To investigate the prevalence of Trichinella infection in field Tibetan pigs, a total of 41 serum samples from Tibetan pigs were examined by ELISA using ES antigen from T. spiralis muscle larvae. The Tibetan pigs were selected from Shuangcha township, Luqu county and Gannan Tibetan Autonomous Prefecture of Gansu province, Northwestern China. All blood samples were collected from the precaval vein of the pigs, and the sera were separated by centrifugation at 1500 × g for 20 min in the State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences. The ELISA results were read by the Model 680 Microplate reader (Bio-Rad) using OD450 nm and analyzed by Microplate Master™ software. The results were evaluated as sample/negative control (S/N) ratio by comparing the mean OD value of the duplicate sample using Excel. An S/N ratio of ≥ 2.1 was regarded as positive.

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