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2.8. Coverslips and Dishes Preparation for Immunofluorescence Assay

JS Juan José Saez
SD Stephanie Dogniaux
MS Massiullah Shafaq-Zadah
LJ Ludger Johannes
CH Claire Hivroz
AZ Andrés Ernesto Zucchetti
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Coverslips of 12mm ø (VWR, 631-0666) for fixed cells or fluorodishes (World Precision Instrument Inc., Sarasota, FL, USA, FD35-100) for live imaging were precoated with 0.02% of poly-L- Lysine for 20 min at room temperature and were washed three times in water before being dried and kept for a maximum of 2 days.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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