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2.4. RNA Extraction and Reverse Transcription
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Total RNA was extracted from 100 mg of tissue homogenised with ceramic beads in a FastPrep-24 5 G tissuelyser in 1 mL of Trizol following the manufacturer’s instructions. Concentration and purity of RNA was estimated using a Nanodrop 2000 C Spectrophotometer. First strand cDNA was synthesised from 1 μg RNA using a Biorad iScript advanced cDNA kit with an integrated genomic DNA elimination step. First strand cDNA samples were diluted 20-fold (working stock) with RNase/DNase free water (Sigma, St. Quentin Fallavier, France) and stored at −20 °C.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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