2.3. Isolation of Linalool from the Purified Essential Oil of Lavender

Linalool was separated from the purified lavender essential oil using normal column chromatography techniques, according to Savira, et al. [22], with some amendments. Silica gel was used as a stationary phase, the sample to stationary phase ratio was 1:20, the sample weight was 10 gm, and the mobile phase was benzene: ethyl acetate (95: 5, v/v). The isocratic elution mode was used at a flow rate of 3 drops per second, and eluent was collected in vials, each at 50 mL. The elution was monitored by thin-layer chromatography (TLC) (Silica gel 254 nm, same mobile phase as the column), and visualization was done using ultraviolet (UV) light at 254 nm and by using anisaldehyde/sulphuric acid reagent or iodine vapor.