4.2. Wound Healing Screening

Huh7 cells were seeded in 96-well plates at 90% confluence and then incubated overnight. A wound was created using a white tip (Axygen Scientific, Inc., Waltham, MA, USA). The cultured medium was sucked and replaced with diluted chemicals obtained from the library. All the chemicals were dissolved in DMSO (Sigma-Aldrich, St. Louis, MO, USA) and diluted to 1 μM each with 1% FBS medium. The scratched cells were treated with the chemicals for 24 h. The cells that migrated into the wound surface were observed using a JuLI Stage Real-Time Cell History Recorder (NanoEnTek, Inc., Seoul, Korea). The change in wound closure is represented as the percentage of wound recovery. All the experiments were performed in triplicate.