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Nur77 was silenced by transfection of Nur77 siRNA (175 nM for NRCMs, 40 nM for CFs; SMARTpool, Dharmacon) using lipofectamine RNAiMAX (Invitrogen). A non-targeting control siRNA pool served as control. Transfections were carried out in serum-free Opti-MEM medium (Gibco, Gaithersburg, MD, USA) for 6 h. Cells were used for experiments 24 h after transfection.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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