2.1. Sample Collection, Morphological Observation, and Fungal Isolation

Samples of submerged decayed wood were collected from a freshwater stream in Chiang Mai Province (19°07.200′ N, 98°44.044′ E) and river in Tak Province in Thailand (17°28′20.7834″ N, 98°1′16.3236″ E) and treated as detailed in Senanayake et al. [29]. The samples were incubated for five days and periodically observed using stereomicroscope to check the presence of fruiting bodies. Micromorphological features were photographed using a Motic SMZ 168 Series dissection microscope for fungal structures on the woody substrate while microscopic characters were documented using Nikon Eclipse 80i microscope-camera system. Tarosoft (R) Image FrameWork was used to measure the micromorphological characters and photographic plates’ preparation was done using Adobe Photoshop CC 2020. The mean size/length of at least 10 ascomata/conidiomata, 20 conidiophores, 20 asci, and 30 conidia/ascospores were measured and recorded. Single-spore isolation was used to obtain pure cultures and colonial characteristics in malt extract agar (MEA) were described. Herbarium type specimens were deposited in Mae Fah Luang University (MFLU). Ex-type living cultures were deposited at Mae Fah Luang University Culture Collection (MFLUCC). The new species were registered in Faces of Fungi (http://http://www.facesoffungi.org/ (accessed on 13 January 2021)) [30] and MycoBank databases (https://www.mycobank.org/ (accessed on 13 January 2021)) [31]