2.10. Glutathione Peroxidase Activity

Glutathione peroxidase (EC 1.11.1.9) activity was measured spectrophotometrically using the “Glutathione Peroxidase Assay Kit” (Abcam, Cambridge CB4 OFL, UK), following manufacturer’s instructions. Glutathione peroxidase (GPX) catalyzes the reduction of H₂O₂ to H₂O via GSH oxidation to GSSG. The decrease in NADPH, proportional to GPX activity, was monitored at 340 nm. The enzymatic activity was expressed as nmol H₂O₂ min⁻¹ per mg of protein.