2.3. Cell Viability Assay

To evaluate the proliferative effect of Ged on B16F10 mouse melanoma cell line, MTS assay was performed according to previously reported protocol [20] using CellTiter 96 Aqueous One Solution Cell Proliferation Assay kit (Promega, Madison, WI, USA). Briefly, 1 × 103 cells were seeded in each well of the 96-well plate and incubated for 24 h for recovery. After incubation, Ged was treated with various concentrations: 0, 3.12, 6.25, 12.5 25, 50, and 75 μM. After 48 h, 20 μL of MTS assay solution was added to each well containing 100-μL media with Ged and without Ged. After an additional incubation for 4 h, absorbance was measured at 490 nm using Multiskan GO spectrophotometer (Thermo Scientific, Waltham, MA, USA). The absorbance data were normalized with the control and represented as a percentage inhibition ratio.