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2.11. Ferric-Reducing/Antioxidant Power (FRAP) Activity
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The FRAP assay was carried out according to previous investigations [27]. Caffeic acid, L-ascorbic, and uric acid were prepared as described above. A standard curve of Trolox (0.034–0.612 mM) was used to quantify FRAP activity. The absorbance of the reagent mixture was measured at 593 nm after 30 min incubation at 25 °C. The results of this assay were calculated considering the data obtained from nine independent assays (n = 9) and showed as Trolox equivalents (mM).
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