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Mitochondrial membrane potential (ΔΨm) measurements
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DLD-1 and SW480 cells were cultivated in P96-well plate and treated with NiCl2 (500µM), ascorbate or both for 2, 4 and 6 hours. ΔΨm determination was carried out using an JC-1 - Mitochondrial Membrane Potential Assay Kit (abcam).
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