The liver tissues from the SD rats in each group were accurately weighed and then homogenized in normal saline. Liver tissue homogenates with a 10% mass fraction were prepared and stored at 4°C. After centrifugation at 3000g for 10 mins, the levels of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) activity as well as the levels of reduced glutathione (GSH) and lipid peroxidation products (LPOs) in the supernatants were determined using assay kits according to instructions provided by the manufacturers.
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