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4.10. Mesangial Expansion

CS Christian Sávio-Silva
PS Poliana E. Soinski-Sousa
AS Antônio Simplício-Filho
RB Rosana M. C. Bastos
SB Stephany Beyerstedt
ÉR Érika Bevilaqua Rangel
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BTBRob/ob and wild type kidney sections (paraffin-fixed, 3–4 µm thick) were stained with periodic acid-Schiff (PAS) trichrome staining in each experimental group. Sections were then analyzed by light microscopy (magnification, 40×). A quantitative analysis of mesangial expansion was performed. The increase in mesangial matrix was determined by the presence of PAS-positive area in the mesangium, and was expressed as a percentage. The glomerular area (μm2) was also traced along the outline of capillary loops using CellSens software (Olympus) in 30 randomly selected glomeruli in each animal.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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