3.3.2. GC-MS

The GC system consisted of an Agilent 7890 gas chromatograph coupled to an Agilent 7000C triple quadrupole detector and to an Agilent 7650C autosampler. MassHunter Acquisition Software B.08.00 version (Agilent Technologies) was used to control the equipment and for data acquisition. Each derivatized sample (0.5 μL) was injected in splitless mode at 280 °C using a multimode inlet (MMI) equipped with an Ultra Inert liner from Agilent. Chromatographic separation was done by using an Agilent HP 5MS capillary column (30 m × 0.25 mm × 0.25 μm). The oven temperature was initially 100 °C (4.0 min hold) and ramped to 300 °C at 4.0 °C min⁻¹ (1.56 min hold). Helium was used as a carrier gas with a flow rate of 1.0 mL min⁻¹. The MS was operated in electron impact ionization mode at 70 Ev and full scan monitoring mode from m/z 35 to 600. The transfer line and ionization source temperatures were set to 280 °C.