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sgRNA sequences were designed using the Cas-Designer (http://www.rgenome.net/cas-designer/) based on the mouse reference genome mm10 [35]. sgRNAs with fewer potential off-target sites and higher microhomology scores were selected to optimize the mutation rate and specificity.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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