2.2.3. Minimum Bactericidal Concentration (MBC) and Minimum Inhibitory Concentration (MIC) Evaluation

For determining the MIC and MBC, samples were serially diluted from their initial concentration (20 mg/mL). In total, 120 μL of bacterial culture (1 × 10⁵ CFUs/mL) were added to a 96-well plate and incubated (37 °C) with 80 µL of each sample. The F. nucleatum plate was sealed with paraffin films to avoid contamination. The MIC value was determined by evaluating the visible growth of microorganisms. For determining the MBC value, 100 μL of culture broth was taken from the well and swabbed into an agar plate. After 24 h of incubation, the MBC value was measured. The experiment was repeated three times and the values of MIC and MBC were given in percentage [39].