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2.8. Transmission Electron Microscopy (TEM)

AR Aida Robles-Fort
IG Inmaculada García-Robles
WF Wasundara Fernando
DH David W. Hoskin
CR Carolina Rausell
MR María Dolores Real
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Samples for TEM analysis were prepared in the same way as for SEM, with the exception of the ethanol graduated dehydration series in which only the 90° gradation was reached. Then, the samples were embedded in resin in 4 steps: 96° ethanol-LR-White 2:1 resin (20 h), 100° ethanol-2:1 resin (20 h), 100° ethanol-1:2 resin (20 h) and 100% resin (24 h at 60 °C). After block preparation, 60 nm-thick ultra-thin sections were cut with a Leica UC6 Ultracut Microtome. The sections were contrasted with lead and the results were observed in the TEM JEOL-JEM1010 equipment at 70 kV, in the Microscopy Service of the Central Research Support Facilities of the University of Valencia.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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