Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

2.17. IncuCyte Single Spheroid Invasion Assay

VC Vanessa Castelli
MC Mariano Catanesi
MA Margherita Alfonsetti
CL Chiara Laezza
FL Francesca Lombardi
BC Benedetta Cinque
MC Maria Grazia Cifone
RI Rodolfo Ippoliti
EB Elisabetta Benedetti
AC Annamaria Cimini
Md Michele d’Angelo
ask Ask a question
Favorite

For the detection of invasion in live cells, mammospheres were seeded (100 µL/well) into a 96-well plate and exposed to GW6471 treatment. Then Matrigel was added on top at a final assay concentration of 50%. Then spheroids were monitored for 72 h in the IncuCyte analyzer. The images acquired were analyzed by the Incucyte ZOOM live-cell analysis system (Essen Bioscience, Newark, UK), and the data were reported as invading cell area, bright field (BF), and area *104 (µm2).

Copyright and License information:  ©2021 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A