4.7. Survival Assay under Juglone-Induced Oxidative Stress

Age-synchronized L1 larvae of wild-type N2 and transgenic CF1038 (DAF-16 loss-of-function mutant) worms were transferred to S-medium containing E. coli OP50 (OD600 = 1.0). They were divided into seven groups. Each group contained 80 worms and treated with the extracts as mentioned before for 48 h at 20 °C. After incubation time, we added pro-oxidant juglone (a naphthoquinone from Juglans regia) to a final concentration 80 µM and incubated the worms at 20 °C for 24 h. The surviving and dead worms were counted.