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2.5. BMP-2 Release Kinetics

KH Kuo-Hao Huang
CW Chen-Ying Wang
CC Cheng-Yu Chen
TH Tuan-Ti Hsu
CL Chun-Pin Lin
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The BMP-2-loaded scaffolds were immersed in Dulbecco’s modified Eagle’s medium (DMEM) for this test. The DMEM was changed at specific time points and assessed for levels of BMP-2 using an enzyme-linked immunosorbent assay kit (Thermo Fisher Scientific, Carlsbad, CA, USA) and a spectrophotometer (TECAN Infinite Pro M200, Männedorf, Switzerland). All experiments were performed in triplicate, with the averages recorded.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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