5.3. Folch Method

The Folch method is another lipid extraction method known for its efficiency in lipid recovery at laboratory scale. The method is almost identical to Bligh and Dyer method, but uses a different ratio of solvents, chloroform:methanol (2:1 v/v), and the protocol includes washing the extract by the addition of a certain amount of salt solution. The mixture of solvents and cells resulted in a separation of two layers, consisting of pure lipid in lower phase and organic solvents in the upper phase [121]. Table 4 summarizes lipid recovery from various oleaginous yeasts extracted by the Folch and the Bligh and Dyer methods after a pretreatment either using a mechanical or nonmechanical method.

Applications of the Bligh and Dyer and the Folch methods for the recovery of intracellular lipids from various oleaginous yeasts.