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The siRNA sequences specific for CXCR4 were: CXCR4–siRNA–1, 5′-CGGGAUACCUACCAUCCUA-3′; CXCR4–siRNA–2, 5′-CAGCUCUACUGAGAAGAAU-3′. According to the manufacturer’s instructions, cells were transfected with siRNA using Lipofectamine™ RNAiMAX reagent (Invitrogen). Briefly, cells were seeded into 6-well plates using opti-MEM supplemented with 10% FBS and were incubated with siRNA for 24–72 h.
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