Clonogenic survival assays

DH Diana van den Heuvel
CS Cornelia G. Spruijt
RG Román González-Prieto
AK Angela Kragten
MP Michelle T. Paulsen
DZ Di Zhou
HW Haoyu Wu
KA Katja Apelt
YW Yana van der Weegen
KY Kevin Yang
MD Madelon Dijk
LD Lucia Daxinger
JM Jurgen A. Marteijn
AV Alfred C. O. Vertegaal
ML Mats Ljungman
MV Michiel Vermeulen
ML Martijn S. Luijsterburg
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Cells were plated in low density in culture dishes, allowed to attach and treated with Illudin S at different concentrations for 72 h. Illudin S was removed and cells were allowed to form clones for 7–10 days. To visualize clones, cells were subjected to NaCl fixation and methylene blue staining. Cell survival after Illudin S treatment was defined as the percentage of cells able to form clones, relative to the untreated condition.

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