Cells were plated in low density in culture dishes, allowed to attach and treated with Illudin S at different concentrations for 72 h. Illudin S was removed and cells were allowed to form clones for 7–10 days. To visualize clones, cells were subjected to NaCl fixation and methylene blue staining. Cell survival after Illudin S treatment was defined as the percentage of cells able to form clones, relative to the untreated condition.
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