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In this assay, RIP assay was performed according to the supplier’s direction of Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Billerica, MA, USA). After being lysed in the complete RIP lysis buffer at 80% confluence, CAL-27 and HSC6 cell extracts were incubated with magnetic beads conjugated with anti-Argonaute 2 antibody (Anti-Ago2; Millipore) or negative control normal mouse IgG (Millipore). After 24 h of incubation, samples were treated with proteinase K, followed by purification and analysis using RT-qPCR assay.
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