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FF Fábio Fernandes
CM Carlos Henrique Valente Moreira
LO Lea Campos Oliveira
MS Marcela Souza-Basqueira
BI Barbara Maria Ianni
CL Claudia di Lorenzo
FR Felix José Alvarez Ramires
LN Luciano Nastari
EC Edecio Cunha-Neto
AR Antonio L. Ribeiro
RL Renato Delascio Lopes
SK Sheila M. Keating
ES Ester Cerdeira Sabino
CM Charles Mady
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From each participant, we collected 20 mL of EDTA-anti-coagulated blood that were immediately mixed with an equal volume of 6 M guanidine hydrochloride -0.2M EDTA solution. The target-capture real-time PCR assay used in this study was developed based on the PCR method described by Virreira et al.,7 targeting the kinetoplast T. cruzi mitochondrial minicircle DNA. The DNA extraction was improved with a target-capture step using magnetic beads coated with a T. cruzi-specific 20-mer capture oligonucleotide.

Copyright and License information:  ©2021-02-19
This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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