Peptide

JZ Jacob A Zahm
MS Michael G Stewart
JC Joseph S Carrier
SH Stephen C Harrison
MM Matthew P Miller
request Request a Protocol
ask Ask a question
Favorite

A 33-mer C-terminal Stu2 peptide, with a selenomethionine in place of the natural methionine at position 876 (EESYKRAAAVTSTLKARIEK(Mse)KAKSRREGTTRT), was synthesized at the Tufts University Core facility. The lyophilized peptide was resuspended to a final concentration of 10 mg/mL in a buffer containing 10 mM TRIS pH 7.0, 100 mM NaCl, and 2 mM TCEP.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A