A 33-mer C-terminal Stu2 peptide, with a selenomethionine in place of the natural methionine at position 876 (EESYKRAAAVTSTLKARIEK(Mse)KAKSRREGTTRT), was synthesized at the Tufts University Core facility. The lyophilized peptide was resuspended to a final concentration of 10 mg/mL in a buffer containing 10 mM TRIS pH 7.0, 100 mM NaCl, and 2 mM TCEP.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.