Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Auxin inducible degradation

JZ Jacob A Zahm
MS Michael G Stewart
JC Joseph S Carrier
SH Stephen C Harrison
MM Matthew P Miller
request Request a Protocol
ask Ask a question
Favorite

The AID system was used essentially as described (Nishimura et al., 2009). Briefly, cells expressed C-terminal fusions of the protein of interest to an auxin responsive protein (IAA7) at the endogenous locus. Cells also expressed TIR1, which is required for auxin-induced degradation. 500 μM IAA (indole-3-acetic acid dissolved in DMSO; Sigma) was added to media to induce degradation of the AID-tagged protein. Auxin was added for 30 min prior to harvesting cells or as is indicated in figure legends.

Copyright and License information:  ©2021, Zahm et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A