Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

2.2. Cloning

AD Anna Dreyer
PT Patrick Treffon
DB Daniel Basiry
AJ Anna Maria Jozefowicz
AM Andrea Matros
HM Hans-Peter Mock
KD Karl-Josef Dietz
request Request a Protocol
ask Ask a question
Favorite

PRX-IIE (AT3G52960), Glutaredoxin-S12 (GRX-S12; AT2G20270), and sulfiredoxin (SRX; AT1G31170) were cloned into pET28a (Novagen, Darmstadt, Germany). Forward and reverse primer were designed with NdeI and BamHI restriction sites, respectively (Table S1). The variants C121S, C146S, C121S/C146S, S82D, T108E, and T223E of PRX-IIE were generated by site-directed mutagenesis with specific primers (Table S1). The correctness of all constructs was confirmed by DNA sequencing.

Copyright and License information:  ©2021 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A