Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

2.8. Evaluation of Biofilm Inhibition

FR Federica Rinaldi
LM Linda Maurizi
AC Antonietta Lucia Conte
MM Massimiliano Marazzato
AM Alessandro Maccelli
MC Maria Elisa Crestoni
PH Patrizia Nadia Hanieh
JF Jacopo Forte
MC Maria Pia Conte
CZ Carlo Zagaglia
CL Catia Longhi
CM Carlotta Marianecci
MA Maria Grazia Ammendolia
MC Maria Carafa
request Request a Protocol
ask Ask a question
Favorite

To measure the biofilm inhibition induced by the SEO and selected NEs, the growth medium was supplemented with 0.02% of Tween-80, then the SEO and NEs were twofold diluted into the medium at sub-MIC concentrations. EO and NE inhibition of cell attachment was evaluated after 48 h incubation at 37 °C. Values higher than 40% were considered significant in biofilm inhibition.

The percentage of biofilm inhibition by sub-MIC EO and NE has been calculated using the following formula [44]:

Copyright and License information:  ©2021 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A