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Yeast Two-Hybrid Assay

JF Jianmei Fu
GL Guoqiang Liu
BL Biao Liu
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A yeast two-hybrid point-to-point verification system based on the Matchmaker Gold yeast two-hybrid system was constructed (Clontech Laboratories, Mountain View, CA, United States). The target genes infused to the AD and BD vectors were transformed into competent Y2H Gold yeast cells. The co-transformed yeast cells were coated onto SD-Trp-Leu-His-Ade medium with X-α-gal for 4 days.

Copyright and License information:  ©2021 Fu, Liu and Liu.
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