CIBERSORT algorithm analysis

CIBERSORT is a deconvolution algorithm that calculates immune cell infiltration proportions on the basis of gene expression profiles in the sample.[18] We used a white blood cell gene signature matrix LM22 that includes 547 genes to identify 22 kinds of infiltrating immune cells. In our current analysis, the CIBERSORT tool was utilized to analyze the infiltrating fractions of immune cells of 505 cases of HNSCC from TCGA. We divided patients into the PRF1^high and PRF1^low group according to the cutoff point (111.0) for PRF1 expression, which was calculated through the automatic Cutoff Finder platform.