Transfection and viral infection

The PDK1 PCR product was ligated using pMX-IRES-puromycin vector with EcoRI and XhoI as restriction enzyme sites. The primers used for cloning are shown in Table S1. The pMX-IRES-puromycin vector (EV) and pMX-IRES-puromycin PDK1 vector (PDK1) were transfected into Plat-A cells using Lipofectamine^™ 2000 (Invitrogen), the supernatant was collected after 24 h, and then it was replaced with the filtered viral supernatant and 5 µg/ml polybrene (sc-134220; Santa Cruz Biotechnology) instead of the 12Z cell culture medium. Twenty-four hours after the infection, the cells were selected using 2 µg/ml puromycin (CAS58-58-2; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and cultured for 2 weeks.