Pulse-chase assay of BODIPY 558/568 C12 (Red C12)

JW Jingru Wang
NF Na Fang
JX Juan Xiong
YD Yuanjiao Du
YC Yue Cao
WJ Wei-Ke Ji
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HEK293 cells were pulsed by an incubation in complete medium (DMEM with 10% fetal bovine serum) containing 1 μM BODIPY 558/568 C12 (ThermoFisher Scientific, D3835) for 16 h. Cells were washed with PBS three times and then chased in EBSS for the time indicated.

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