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Arabidopsis thaliana Col-0 plants as well as single mutant lines (sus1-6 and cinv1-2) and multiple mutant lines (sus1/2/3/4 and cinv1/2) were analyzed at 10 dai. Shoot fresh and dry weight was determined using an analytical balance model 770 (Kern & Sohn GmbH, Balingen, Germany). For analysis of dry shoot weight, shoots were harvested and dried for 3 h at 90 °C in a UF 260 plus (Memmert GmbH, Schwabach, Germany). Main root length was assessed with a ruler, and the number of side roots per main root length was calculated.
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