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Synthesis of BHMF by a Fed-Batch Strategy

SC Siyuan Chang
XH Xuejun He
BL Bingfeng Li
XP Xin Pan
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The reaction mixture was incubated at 35°C and 180 rpm, containing 50 mM pH 7 PBS, 100 mM HMF, co-substrate (70 mM glutamine, 30 mM sucrose), 1.0 mM Mn2+, and 20 mg/mL (wet weight) B. contaminans NJPI-15 resting cells. When the HMF was almost used up, 100 mM HMF and co-substrates were supplied. After 33 h, the cells were separated from the reaction mixture and added to a fresh mixture of 50 mM PBS, 100 mM HMF and co-substrates for the next round.

Copyright and License information:  ©2021 Chang, He, Li and Pan.
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